Many of us have had the experience of trying to reconstruct what someone has done and been frustrated trying to find the exact sequence. Relative coordinates do not last: gene models often change so that “Leu234” in a protein is no longer there and our knowledge of genome sequence changes (or we are working with a different strains) so the EcoR1 site 5’ to your favorite gene is not there. There is an easy solution: always specify a location by sequence. Thirty nucleotides is sufficient in essentially all cases to uniquely locate the site. Your simple effort in specifying a genome location by sequence, when you are writing a paper will make experiments easily reproducible, as well as help WormBase in curating such studies.
WormBase requests that authors provide complete information about genetic entities such as strains, alleles, transgenes, etc., in published papers. Providing a clear list of the experimental genetic entities used, in the paper, along with complete information about them would make curating your paper easier and quicker, saving time and effort that curators spend in searching for such information and/or writing to authors. For example, if you use strains, please provide the complete list of strain names and their genotypes, including transgenes, markers and any additional components. Papers with incomplete information about genetic entities and reagents can only be partially curated or not curated at all, making valuable data about worm models of biology and disease unavailable to both the worm and biomedical research communities.
Check out the latest chapter of WormBook in Genetics-‘Invading, Leading and Navigating Cells in Caenorhabditis elegans: Insights into Cell Movement in Vivo‘, by David R. Sherwood and Julie Plastino.
If you are looking for interaction data for C. elegans look no further than our FTP site. At every release WormBase deposits data files on the FTP site under the relevant release number and species directories. The files are named after the data they contain. The interactions file for C. elegans for the WS261 release can be found here and is called c_elegans.PRJNA13758.WS261.interactions.txt.gz
The latest chapter of WormBook is now available: Cell Biology of the Mitochondrion by Alexander M. van der Bliek, Margaret M. Sedensky and Phil G. Morgan.