Specify genome locations with 30 nucleotides of flanking sequence!

Many of us have had the experience of trying to reconstruct what someone has done and been frustrated trying to find the exact sequence. Relative coordinates do not last: gene models often change so that “Leu234” in a protein is no longer there and our knowledge of genome sequence changes (or we are working with a different strains) so the EcoR1 site 5’ to your favorite gene is not there.  There is an easy solution: always specify a location by sequence. Thirty nucleotides is sufficient in essentially all cases to uniquely locate the site. Your simple effort in specifying a genome location by sequence, when you are writing a paper will make experiments easily reproducible, as well as help WormBase in curating such studies.

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