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In the framework of the European Union funded Nemagenetag project, several labs worked together from 2003 to 2007 to generate a genome-wide collection of molecularly characterized Mos1 insertion mutants for the C. elegans community. The relevance and utility of this collection of >13000 strains has been drastically reduced with the advent of CRISPR. Requests for strains have dropped to an average of less than one a month. The Nemagenetag members consider that the cost involved in maintaining the collection is no longer warranted. Any laboratory interested in the collection (available in duplicate) should contact us before 15th June. If there are no takers, we will dispose of the collection as we see fit.
We take this opportunity to remind you that if ever you use a Nemagenetag allele (“ttTi”, including MoSCI alleles such as ttTi5605) you should always cite the relevant publications. Without such citation, community projects will not get funding.
Vallin et al.
A genome-wide collection of Mos1 transposon insertion mutants for the C. elegans research community.
PLoS One. 2012;7(2):e30482. doi: 10.1371/journal.pone.0030482. Epub 2012 Feb 8.
Duverger et al.
A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode Caenorhabditis elegans.
Nucleic Acids Res. 2007;35(2):e11. Epub 2006 Dec 12.
Jonathan Ewbank on behalf of the Nemagenetag consortium http://elegans.imbb.forth.gr/nemagenetag/
Check out the chapter ‘The Genetics of Axon Guidance and Axon Regeneration in Caenorhabditis elegans‘ from WormBook in GENETICS, by
Check out the latest chapter of WormBook in Genetics, Cell Biology of the Caenorhabditis elegans Nucleus, by Orna Cohen-Fix and Peter Askjaer.
Check out the latest chapter in WormBook in GENETICS–‘Next-Generation Sequencing-Based Approaches for Mutation Mapping and Identification in Caenorhabditis elegans‘ by Maria Doitsidou, Sophie Jarriault and Richard J. Poole.