Nuclear receptor chapter updated in WormBook

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Nuclear receptor signal transduction in C. elegans, by Adam Antebi, has just been published in the Signal transduction section of WormBook. This chapter updates his 2006 chapter, Nuclear hormone receptors in C. elegans. The updated chapter discusses the role of nuclear receptors as regulators of life history, cell fate, and organogenesis, and their function in nutrient sensing, metabolism and energy homeostasis.

Please proceed to read this new chapter and others on nematode biology at http://www.wormbook.org/. If you have any comments or suggestions, please submit them via the Feedback page on wormbook.org.

Thank you for your interest in WormBook.

Jane

WS248: Parasite Papers II

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For the upcoming WS248 release, a set of new papers have been added to the WormBase database. Some papers of interest to the parasite community are shown below.

Doharey PK, Suthar MK, Verma A, Kumar V, Yadav S, Balaramnavar VM, Rathaur S, Saxena AK, Siddiqi MI, Saxena JK. Molecular cloning and characterization of Brugia malayi thymidylate kinase. Acta Trop. 2014 May;133:83-92. doi:10.1016/j.actatropica.2014.02.003. Epub 2014 Feb 17.

genes referred to: Bm5401

Singh VK, Doharey PK, Kumar V, Saxena JK, Siddiqi MI, Rathaur S, Narender T.
Synthesis, molecular docking and Brugia malayi thymidylate kinase (BmTMK) enzyme inhibition study of novel derivatives of [6]-shogaol. Eur J Med Chem. 2015 Mar 26;93:74-82. doi: 10.1016/j.ejmech.2015.01.035. Epub 2015 Jan 20.

genes referred to: Bm5401

Bulman CA, Bidlow CM, Lustigman S, Cho-Ngwa F, Williams D, Rascón AA Jr, Tricoche N, Samje M, Bell A, Suzuki B, Lim KC, Supakorndej N, Supakorndej P, Wolfe AR, Knudsen GM, Chen S, Wilson C, Ang KH, Arkin M, Gut J, Franklin C,
Marcellino C, McKerrow JH, Debnath A, Sakanari JA. Repurposing auranofin as a lead candidate for treatment of lymphatic filariasis and onchocerciasis. PLoS Negl Trop Dis. 2015 Feb 20;9(2):e0003534. doi: 10.1371/journal.pntd.0003534. eCollection 2015 Feb.

genes referred to: Bma-trxr-1

WS248: Parasite Papers I

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For the upcoming WS248 release, a set of new papers have been added to the WormBase database. Some papers of interest to the parasite community are shown below.

Singh AR, Joshi S, Arya R, Kayastha AM, Srivastava KK, Tripathi LM, Saxena JK. Molecular cloning and characterization of Brugia malayi hexokinase. Parasitol Int. 2008 Sep;57(3):354-61. doi: 10.1016/j.parint.2008.03.004. Epub 2008 Apr 9.

genes referred to: Bm4678

Poole CB, Davis PJ, Jin J, McReynolds LA. Cloning and bioinformatic identification of small RNAs in the filarial nematode, Brugia malayi. Mol Biochem Parasitol. 2010 Feb;169(2):87-94. doi: 10.1016/j.molbiopara.2009.10.004. Epub 2009 Oct 27.

linked miRNA genes can be found at the WBPaper00046326 page

Singh N, Heneberg P, Singh N, Singh SK, Rathaur S. Identification of a novel stress regulated FERM domain containing cytosolic protein having PTP activity in Setaria cervi, a bovine filarial parasite. Biochem Biophys Res Commun. 2015 Feb 27;458(1):194-200. doi: 10.1016/j.bbrc.2015.01.100. Epub 2015 Jan 31.

genes referred to: Bma-ptp-1 , ptp-1

Devarajan E, Mishra PK, Thirugnanam S, Mehta K, Chandrashekar R, Perumal K. Molecular characterization of a Brugia malayi transglutaminase. Parasitol Res. 2004 Jun;93(2):145-50. Epub 2004 May 1.

genes referred to: Bma-pdi-3

WormBook: Operon chapter added

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Dear WormBook reader,

Operon and non-operon gene clusters in the C. elegans genome, by Thomas Blumenthal, Paul Davis and Alfonso Garrido-Lecca, has been published in the Molecular biology section of WormBook. This chapter discusses a variety of tight-linkage gene arrangements in the C. elegans genome, summarizes the current knowledge on several variations of operon processing, and describes non-operon gene clusters.

Please proceed to read this new chapter and others on nematode biology at http://www.wormbook.org/. If you have any comments or suggestions, please submit them via the Feedback page on wormbook.org.

Thank you for your interest in WormBook.

Jane

CRISPR workshop at the 2015 C.elegans meeting

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CRISPR-based Strategies for Genome Engineering
Announcing the “CRISPR revolution” workshop to be held during the final plenary session of the 2015 International C. elegans Meeting,
June 28th, 10:30am – 12:00pm, Royce Hall Auditorium
Organizers: Mike Boxem, Daniel Dickinson, Alexandre Paix

CRISPR is a rapidly evolving technology that is quickly becoming an essential tool for every C. elegans lab. We would like to bring together representatives of the various groups that are developing CRISPR-based approaches for C. elegans to discuss current strategies for genome modifications. Specific topics to be discussed will include screening strategies, improving efficiency, target site selection, and guidelines for making different kinds of modifications. The workshop will conclude with an overview by Geraldine Seydoux of the status of CRISPR-based methods in C. elegans, based on previous reports and current methods presented in the workshop or elsewhere at the meeting.

For members of the community who are actively developing CRISPR methods, the workshop will serve as an opportunity to compare notes, share ideas, and stay current on the efforts of others. For those who are “end users” of these methods, it will be an opportunity to learn the latest approaches, obtain protocols, ask questions and seek advice.

Researchers who actively develop CRISPR-based approaches or are making technical improvements are invited to share their results and experiences. If you would like to speak at this workshop, please submit your abstract under the category “V, Gene Regulation and Genomics, Novel Genetic Technologies” to the 2015 C. elegans Meeting.

If you have questions about this workshop, contact Mike Boxem.