Enhanced C. elegans RNAi library – supplementary clones now available to order from Source BioScience

The C.elegans RNAi library has been extended with 3507 new supplementary bacterial clones and now targets around 87% of currently annotated C.elegans genes. Purchase the complete set of all clones or individual chromosome sets (I, II, III, IV, V and X) which include the pre-existing and new supplemental strains. The complete supplementary sets of RNAi clones and individual chromosome suplementary sets (I, II, III, IV, V and X) are also available to order for those who have previously purchased the pre-existing library.

Order now to complete your sets

Improving the C.elegans genome

We have been working on marking possible genome sequence errors in the C.elegans reference genome using data from the following projects:

– Weber KP et al. (2010) PLoS One “Whole genome sequencing highlights genetic changes associated with laboratory ….” (PMID 21085631)

– Doitsidou M et al. (2010) PLoS One “C. elegans mutant identification with a one-step whole-genome-sequencing and ….” (PMID 21079745)

– McGrath PT et al. (2011) Nature “Parallel evolution of domesticated Caenorhabditis species targets pheromone ….” (PMID 21849976)

This data has been used to mark regions for improvement (also available as GFF3 features), taking the total of these locations in the C. elegans database to 2,428.

We will address some of them in the next WormBase release WS234.

Update: the genome changes were deferred from WS234 to WS235